Gene name |
SPBC2A9.11c |
Gene ID |
22/G03 |
Gene synonyms/obsolete |
SPBC2D10.01c |
Gene product |
conserved eukaryotic
protein; involved in nuclear export; SAC3/GANp family |
Entry clone |
Cloned in 2004
trial |
ORF length (unspliced) |
1306 |
ORF length (spliced) |
1188 |
Entry clone length |
1306 |
No. of intron |
2 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC2A9.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAAAAGAACATCATAG |
Rev primer name |
SPBC2A9.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGATTTGGCCTTTAATATCA |
Amino acid length |
395 |
Molecular weight |
46.2 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
75 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPVLKQTLEL |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |