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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC354.01
Gene ID 22/G02
Gene synonyms/obsolete gtp1; SPBC649.06
Gene product GTP-binding protein; GTP1/OBG family; non-essential
Entry clone Cloned
ORF length (unspliced) 1305
ORF length (spliced) 1092
Entry clone length 1305
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC354.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTGTCTTAGAAAAAGT
Rev primer name SPBC354.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTGTCACAATAGTAACC
Amino acid length 363
Molecular weight 40.7
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKERIWEELNL
Localization (YFP) nucleus>cytosol; microtubules; spindle microtubules
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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