Gene name |
SPAC1F3.07c |
Gene ID |
22/C09 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
1286 |
ORF length (spliced) |
1212 |
Entry clone length |
1286 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
153A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1F3.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGCTGAAAGTTGCTC |
Rev primer name |
SPAC1F3.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCTACGTGATTTTGTAGTC |
Amino acid length |
403 |
Molecular weight |
44.6 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNKLLQHLQL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|