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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC824.09c
Gene ID 22/C07
Gene synonyms/obsolete
Gene product GTPase activating protein; ArfGap domain
Entry clone Cloned
ORF length (unspliced) 1285
ORF length (spliced) 963
Entry clone length 1285
No. of intron 4
Sequence status Finished
Sequence results 160A:G / 515A:G / 531A:G / 570C:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC824.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATAAATTCAAAAAGCAA
Rev primer name SPAC824.09.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCCAAACATCATGAGAA
Amino acid length 320
Molecular weight 35.1
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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