| Gene name |
SPAC144.10c |
| Gene ID |
21/D06 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in GPI anchor
biosynthesis |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
1380 |
| ORF length (spliced) |
|
| Entry clone length |
1380 |
| No. of intron |
0 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC144.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATACAAATTGGAAAA |
| Rev primer name |
SPAC144.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGGCGAAAACGAATTCCG |
| Amino acid length |
459 |
| Molecular weight |
52.1 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
|
| No. of transmembrane domain |
13 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWNSFILLIL/LLFPLCILLVL/LTIMTMYLFI |
| Localization (YFP) |
no expression clone
|
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|