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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC15A10.04c
Gene ID 21/D05
Gene synonyms/obsolete zpr1
Gene product zinc finger protein; possibly binds to translation elongation factor
Entry clone Cloned
ORF length (unspliced) 1380
ORF length (spliced)
Entry clone length 1380
No. of intron 0
Sequence status Finished
Sequence results 154A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC15A10.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGGAAGAAAAAAAGGA
Rev primer name SPAC15A10.04.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCTTACCTCCATCCTTT
Amino acid length 459
Molecular weight 51.3
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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