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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16E8.06c
Gene ID 21/B05
Gene synonyms/obsolete
Gene product RNA-binding protein; rrm RNA recognition motif
Entry clone Cloned
ORF length (unspliced) 1362
ORF length (spliced) 1317
Entry clone length 1362
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC16E8.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCGAAACAAATTCATC
Rev primer name SPAC16E8.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTTATCTTTTTTTCCAGCC
Amino acid length 438
Molecular weight 49.3
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 407
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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