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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC823.16c
Gene ID 19/G02
Gene synonyms/obsolete
Gene product conserved hypothetical; WD repeat protein
Entry clone Cloned
ORF length (unspliced) 1236
ORF length (spliced) 1008
Entry clone length 1236
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC823.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCATCGATTATCCTTTA
Rev primer name SPAC823.16.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGTCAAAGCTAGATATG
Amino acid length 335
Molecular weight 37.1
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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