Gene name |
SPAC23D3.01 |
Gene ID |
19/E01 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; PWWP domain protein; involved in cell growth;
similar to Sp SPBC215.07C and SPBC29A3.13C (paralogs) |
Entry clone |
Cloned |
ORF length (unspliced) |
1224 |
ORF length (spliced) |
|
Entry clone length |
1224 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23D3.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGGTTGCTAGGACACG |
Rev primer name |
SPAC23D3.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTAGTGATGACAGATGGT |
Amino acid length |
407 |
Molecular weight |
46.8 |
Isoelectric point (calc.) |
10.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |