Gene name |
SPCC1393.02c |
Gene ID |
19/D10 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1221 |
ORF length (spliced) |
|
Entry clone length |
1221 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
696T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1393.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGGAACCCCATCATT |
Rev primer name |
SPCC1393.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTATTTTTCCTTCTTTTT |
Amino acid length |
406 |
Molecular weight |
45.6 |
Isoelectric point (calc.) |
10.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
399/393 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus;
spindle microtubules |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus) |
Microscope used for
observation |
DeltaVision |