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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1393.02c
Gene ID 19/D10
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1221
ORF length (spliced)
Entry clone length 1221
No. of intron 0
Sequence status Finished
Sequence results 696T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1393.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGGAACCCCATCATT
Rev primer name SPCC1393.02.Rv
Rev primer SEQ AGAAAGCTGGGTATTTATTTTTCCTTCTTTTT
Amino acid length 406
Molecular weight 45.6
Isoelectric point (calc.) 10.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 399/393
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus; spindle microtubules
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus)
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images
LMB 4 images) See all images

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