| Gene name |
SPBC428.02c |
| Gene ID |
18/A10 |
| Gene synonyms/obsolete |
eca39;
SPBC582.12c |
| Gene product |
branched chain amino
acid aminotransferase; phosphate dependent; involved in
leucine biosynthesis; involved in valine biosynthesis;
involved in isoleucine biosynthesis; non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1143 |
| ORF length (spliced) |
|
| Entry clone length |
1143 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC428.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTCAAACTGCTGCTCT |
| Rev primer name |
SPBC428.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGCAAAGCAGGAACGCTC |
| Amino acid length |
380 |
| Molecular weight |
42.5 |
| Isoelectric point (calc.) |
7.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|