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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC9G1.11c
Gene ID 18/A09
Gene synonyms/obsolete spn4
Gene product septin
Entry clone Cloned
ORF length (unspliced) 1143
ORF length (spliced)
Entry clone length 1143
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC9G1.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGAAGAAGAGACAAA
Rev primer name SPAC9G1.11.Rv
Rev primer SEQ AGAAAGCTGGGTAACGTTTGCGAGAGCTGGTA
Amino acid length 380
Molecular weight 44.7
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol; periphery at site of septum formation
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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