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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC550.04c
Gene ID 17/H05
Gene synonyms/obsolete
Gene product phosphatidylinositol-glycan-class c; involved in GPI anchor biosynthesis
Entry clone Cloned
ORF length (unspliced) 1135
ORF length (spliced) 975
Entry clone length 1135
No. of intron 3
Sequence status Finished
Sequence results 516A:G / 631T:C / 1059T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC550.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGAAGTATGTGCTGC
Rev primer name SPCC550.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGCCCTTACTTGGACCTAGC
Amino acid length 324
Molecular weight 36.6
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain 6
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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