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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6F6.11c
Gene ID 17/H04
Gene synonyms/obsolete
Gene product pyridoxal kinase
Entry clone Cloned
ORF length (unspliced) 1135
ORF length (spliced) 930
Entry clone length 1135
No. of intron 4
Sequence status Finished
Sequence results 175A:G / 667G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6F6.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATACAACAAAGCGTAT
Rev primer name SPAC6F6.11.Rv
Rev primer SEQ AGAAAGCTGGGTAATAATAGTATACTGCTTCG
Amino acid length 309
Molecular weight 33.9
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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