Gene name |
SPBC16A3.19 |
Gene ID |
13/H02 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
similar to D. melanogaster CG13746; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
971 |
ORF length (spliced) |
819 |
Entry clone length |
971 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
558C:T / 957A:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16A3.19.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTCGAGGGGAACAAG |
Rev primer name |
SPBC16A3.19.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTCGGAGGCCTCCGGCTT |
Amino acid length |
272 |
Molecular weight |
30.9 |
Isoelectric point (calc.) |
10.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |