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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16A3.19
Gene ID 13/H02
Gene synonyms/obsolete
Gene product hypothetical protein; similar to D. melanogaster CG13746; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 971
ORF length (spliced) 819
Entry clone length 971
No. of intron 2
Sequence status Finished
Sequence results 558C:T / 957A:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC16A3.19.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTTCGAGGGGAACAAG
Rev primer name SPBC16A3.19.Rv
Rev primer SEQ AGAAAGCTGGGTAGGTCGGAGGCCTCCGGCTT
Amino acid length 272
Molecular weight 30.9
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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