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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC2H8.04
Gene ID 11/F02
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 865
ORF length (spliced) 801
Entry clone length 865
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC2H8.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCGCAGCTTGGCGGAA
Rev primer name SPCC2H8.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGTGAAGAGCGTCGAGAT
Amino acid length 266
Molecular weight 31.1
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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