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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP7G5.01
Gene ID 10/A12
Gene synonyms/obsolete SPAC15E1.10
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 798
ORF length (spliced)
Entry clone length 798
No. of intron 0
Sequence status Finished
Sequence results 329A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP7G5.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAACCCGACTTCCGA
Rev primer name SPAP7G5.01.Rv
Rev primer SEQ AGAAAGCTGGGTACATAAACATATCGGATGAA
Amino acid length 265
Molecular weight 29.5
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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