| Gene name |
SPBC1778.08c |
| Gene ID |
09/B02 |
| Gene synonyms/obsolete |
|
| Gene product |
ARP2/3
actin-organizing complex; involved in actin cortical patch
assembly; involved in actin assembly and function |
| Entry clone |
Cloned |
| ORF length (unspliced) |
747 |
| ORF length (spliced) |
525 |
| Entry clone length |
747 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1778.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCGGTATGTTCTAAAGT |
| Rev primer name |
SPBC1778.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAAGCTTTGTTCATAAAA |
| Amino acid length |
174 |
| Molecular weight |
19.8 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus;
cytoplasmic dots at cell tip and site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |