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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16C4.05
Gene ID 08/E10
Gene synonyms/obsolete
Gene product RNase P and RNase MRP subunit
Entry clone Cloned
ORF length (unspliced) 721
ORF length (spliced) 606
Entry clone length 721
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16C4.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAAAAAGCAAACGAA
Rev primer name SPCC16C4.05.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTTAAACTCGTTTCCC
Amino acid length 201
Molecular weight 22.5
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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