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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23C11.08
Gene ID 08/D01
Gene synonyms/obsolete php3
Gene product CCAAT-box binding factor; required for growth on non-fermentable carbon sources
Entry clone Cloned
ORF length (unspliced) 708
ORF length (spliced) 351
Entry clone length 708
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23C11.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCGCAGACGGGTTGGA
Rev primer name SPAC23C11.08.Rv
Rev primer SEQ AGAAAGCTGGGTACTGAGGCTCTTCGGACCGG
Amino acid length 116
Molecular weight 12.9
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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