Gene name |
SPCC126.11c |
Gene ID |
07/E12 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; RNA-binding protein; no apparent Sc ortholog;
RRM RNA recognition motif |
Entry clone |
Cloned |
ORF length (unspliced) |
654 |
ORF length (spliced) |
552 |
Entry clone length |
654 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC126.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAAAACGAGGCAACAA |
Rev primer name |
SPCC126.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTCTTTTGAGTTCTTCTC |
Amino acid length |
183 |
Molecular weight |
19.7 |
Isoelectric point (calc.) |
11 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear dots;
nucleolus? |
Comments for localization |
bright dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss, DeltaVision
|