| Gene name |
SPBP35G2.09 |
| Gene ID |
07/E11 |
| Gene synonyms/obsolete |
|
| Gene product |
U1 snRNP component;
involved in mRNA splicing; involved in spliceosome assembly
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
654 |
| ORF length (spliced) |
549 |
| Entry clone length |
654 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP35G2.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAAGATGTATGATACG |
| Rev primer name |
SPBP35G2.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCACGTACAAACCTTTGA |
| Amino acid length |
182 |
| Molecular weight |
20.4 |
| Isoelectric point (calc.) |
9.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol; a few nuclear dots |
| Comments for localization |
cytoplasmic aggregates
by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss, DeltaVision
|