| Gene name |
SPBC530.03c |
| Gene ID |
07/A05 |
| Gene synonyms/obsolete |
bag102; bag1-b |
| Gene product |
ubiquitin family
protein; BAG-family; chaperone regulator-1B; similar to Sp
SPBC16G5.11c; YIL016W does not have N-terminal ubiquitin
domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
621 |
| ORF length (spliced) |
|
| Entry clone length |
621 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC530.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTTTTTACCCAGTT |
| Rev primer name |
SPBC530.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTGTTTTGATTCTTGGTG |
| Amino acid length |
206 |
| Molecular weight |
23.3 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision
|