Gene name |
SPBP35G2.12 |
Gene ID |
07/A03 |
Gene synonyms/obsolete |
|
Gene product |
nucleoside
diphosphate-sugar hydrolase; Nudix family hydrolase |
Entry clone |
Cloned |
ORF length (unspliced) |
618 |
ORF length (spliced) |
|
Entry clone length |
618 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
443A:G / 571A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP35G2.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCCAAGATCAAAAACC |
Rev primer name |
SPBP35G2.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGACGACAAGTATTTAAGA |
Amino acid length |
205 |
Molecular weight |
23 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol
|
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |