| Gene name |
SPBC1604.10 |
| Gene ID |
05/H06 |
| Gene synonyms/obsolete |
srb7 |
| Gene product |
RNA polymerase II
holoenzyme component; mediator complex subunit; involved in
regulating RNA polymerase II activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
563 |
| ORF length (spliced) |
417 |
| Entry clone length |
563 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1604.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATGTAGGTGTACTCA |
| Rev primer name |
SPBC1604.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGGCTGAAATAGGACACAT |
| Amino acid length |
138 |
| Molecular weight |
15.8 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |