Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6B12.13
Gene ID 05/E02
Gene synonyms/obsolete
Gene product hypothetical protein; Sc YFR003C may physically interact with Glc7p and Ppz1p; Sc YFR003C is null lethal
Entry clone Cloned
ORF length (unspliced) 540
ORF length (spliced) 315
Entry clone length 540
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6B12.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACTCACAAGACCGTT
Rev primer name SPAC6B12.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCGCGTTCATATGCATTG
Amino acid length 104
Molecular weight 11.6
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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