Gene name |
SPAC13D6.02c |
Gene ID |
05/E01 |
Gene synonyms/obsolete |
byr3 |
Gene product |
zinc finger protein;
zf-CCHC type (zinc knuckle); involved in mating pheromone
recognition pathway; deletion mutant results in conjugation
defects; involved in conjugation; cellular nucleic acid
binding protein homolog; overexpression suppresses sporulation
defects of ras1 mutant |
Entry clone |
Cloned |
ORF length (unspliced) |
540 |
ORF length (spliced) |
|
Entry clone length |
540 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13D6.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGTCTGAATCTGTTCC |
Rev primer name |
SPAC13D6.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCCTCAATTACAGGAGAG |
Amino acid length |
179 |
Molecular weight |
19.3 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |