Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC24C9.16c
Gene ID 05/A03
Gene synonyms/obsolete cox8
Gene product putative cytochrome c oxidase VIIC subunit
Entry clone Cloned
ORF length (unspliced) 508
ORF length (spliced) 201
Entry clone length 508
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC24C9.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTCGTTACAGTCTTCA
Rev primer name SPAC24C9.16.Rv
Rev primer SEQ AGAAAGCTGGGTACAAATTACGGTTATGCTAA
Amino acid length 66
Molecular weight 7.5
Isoelectric point (calc.) 11.6
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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