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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F12.02c
Gene ID 05/A02
Gene synonyms/obsolete p23fy
Gene product conserved eukaryotic protein; translationally controlled tumour protein homolog; potential target of the antimalarial drug artemesinin; TCTP family protein; calcium binding protein; widely-expressed
Entry clone Cloned
ORF length (unspliced) 507
ORF length (spliced)
Entry clone length 507
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F12.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTGTTGTACAAGGACGT
Rev primer name SPAC1F12.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGAACTTCTCAGAAACAAGA
Amino acid length 168
Molecular weight 19
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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