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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP23A10.02
Gene ID 04/D09
Gene synonyms/obsolete
Gene product hypothetical protein
Entry clone Cloned
ORF length (unspliced) 479
ORF length (spliced) 399
Entry clone length 479
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP23A10.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTATTGGGTGGAACT
Rev primer name SPBP23A10.02.Rv
Rev primer SEQ AGAAAGCTGGGTAACTATTATTGGCAGTTGTA
Amino acid length 132
Molecular weight 14.6
Isoelectric point (calc.) 5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVAVFLGLYI/LFFLAICLWL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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