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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H3.07c
Gene ID 03/B08
Gene synonyms/obsolete
Gene product mitochondrial ribosomal protein S14
Entry clone Cloned
ORF length (unspliced) 408
ORF length (spliced) 318
Entry clone length 408
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H3.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGGGCTTTGGGCCACCT
Rev primer name SPAC23H3.07.Rv
Rev primer SEQ AGAAAGCTGGGTACCATGATGCCTTGCTGAAT
Amino acid length 105
Molecular weight 12.2
Isoelectric point (calc.) 11.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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