Gene name |
SPBC16E9.15 |
Gene ID |
03/A05 |
Gene synonyms/obsolete |
|
Gene product |
small protein
conserved in human and D. melanogaster; heat shock factor
binding protein; involved in heat shock response (regulation);
no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
398 |
ORF length (spliced) |
228 |
Entry clone length |
398 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16E9.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGCGTCTGTTAGAGG |
Rev primer name |
SPBC16E9.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCGGTCGATGTGACCGGT |
Amino acid length |
75 |
Molecular weight |
8.3 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|