Gene name |
SPCC285.12 |
Gene ID |
03/A03 |
Gene synonyms/obsolete |
lsm7 |
Gene product |
U6 snRNA-associated
protein; involved in mRNA splicing; Sm domain |
Entry clone |
Cloned |
ORF length (unspliced) |
396 |
ORF length (spliced) |
342 |
Entry clone length |
396 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
(+1)T:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC285.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCAGTCTTCAAAAACG |
Rev primer name |
SPCC285.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCAGCTTGGACGAATGGA |
Amino acid length |
113 |
Molecular weight |
12.4 |
Isoelectric point (calc.) |
5.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTGAIRKLGL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |