| Gene name |
SPCC1322.15 |
| Gene ID |
01/H10 |
| Gene synonyms/obsolete |
rpl3402; rpl34-2 |
| Gene product |
60S ribosomal protein
L34 |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
336 |
| ORF length (spliced) |
|
| Entry clone length |
336 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1322.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTCAAAGAGTTACTTA |
| Rev primer name |
SPCC1322.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTTTGAGACATCTGCTTC |
| Amino acid length |
111 |
| Molecular weight |
12.8 |
| Isoelectric point (calc.) |
11.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol;
nucleolus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to: nucleolus;
nucleus>cytosol; cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |