Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3G6.13c
Gene ID 01/A03
Gene synonyms/obsolete rpl4101; rpl41-1
Gene product 60S ribosomal protein L41
Entry clone Cloned
ORF length (unspliced) 130
ORF length (spliced) 78
Entry clone length 130
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3G6.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTGATAAGTGGAGGAA
Rev primer name SPAC3G6.13.Rv
Rev primer SEQ AGAAAGCTGGGTATTTACTAATTCAAAGTTAG
Amino acid length 25
Molecular weight 3.4
Isoelectric point (calc.) 13.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 10/12/5/14/13
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization changed to: nucleus>>cytosol (intranuclear microtubule bundle?)
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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