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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC11C11.12
Gene ID 01/A02
Gene synonyms/obsolete
Gene product possible pseudogene; similar to part of protein in cob intron
Entry clone Cloned
ORF length (unspliced) 99
ORF length (spliced)
Entry clone length 99
No. of intron 0
Sequence status Finished
Sequence results 24A:C / 36C:T / 56G:A
Comments Originally marked but disappeared.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC11C11.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTATCTAAACGATCAAA
Rev primer name SPBC11C11.12.Rv
Rev primer SEQ AGAAAGCTGGGTAGGCAATAAGGAACATATCT
Amino acid length 32
Molecular weight 3.9
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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