Gene name |
SPCC1450.08c |
Gene ID |
51/F04 |
Gene synonyms/obsolete |
wtf16; wtf8 |
Gene product |
wtf element |
Entry clone |
Cloned |
ORF length (unspliced) |
1308 |
ORF length (spliced) |
1050 |
Entry clone length |
1308 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC285.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAATAATTACACTTC |
Rev primer name |
SPCC285.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGACTTCGCTTTCGGCCTCC |
Amino acid length |
349 |
Molecular weight |
39.9 |
Isoelectric point (calc.) |
6 |
Signal SEQ |
|
No. of transmembrane domain |
6 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSWFCASLAL |
Localization (YFP) |
vacuole |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |