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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC70.06b
Gene ID 51/D04
Gene synonyms/obsolete
Gene product putative leucine permease transcriptional
Entry clone Cloned
ORF length (unspliced) 1272
ORF length (spliced) 1239
Entry clone length 1272
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC70.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGACTAAAGTTAAGCA
Rev primer name SPCC70.06b.Rv
Rev primer SEQ AGAAAGCTGGGTAACTAACTACAATCATACCT
Amino acid length 412
Molecular weight 47
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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