| Gene name |
SPBP22H7.07 |
| Gene ID |
50/F11 |
| Gene synonyms/obsolete |
prp5; cwf1; pi024;
SPACTOKYO_453.10 |
| Gene product |
WD repeat protein;
prl1/prl2 phosphatases pleiotrophic regulator-like; involved
in mRNA splicing (IGI); interacts physically with Cdc5p;
nineteen complex (Ntc)(putative); essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1422 |
| ORF length (spliced) |
|
| Entry clone length |
1422 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP22H7.07.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGACAGAAGCAAAAAACAT |
| Rev primer name |
SPBP22H7.07.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAATATCGCCGACGAAGATTT |
| Amino acid length |
473 |
| Molecular weight |
52.4 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |