| Gene name |
SPAC12B10.12c |
| Gene ID |
49/C12 |
| Gene synonyms/obsolete |
rhp41; rhp4a |
| Gene product |
involved in DNA
repair; involved in nucleotide-excision repair; XP-C family;
repairosome; similar to Sp rhp42 (paralog) |
| Entry clone |
Cloned#/2004.10 &
Cloned#/ 3' FS |
| ORF length (unspliced) |
1917 |
| ORF length (spliced) |
|
| Entry clone length |
1917 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC12B10.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGAGTGACGAAGATAA |
| Rev primer name |
SPAC12B10.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACCGTATTCTTCGAATACA |
| Amino acid length |
638 |
| Molecular weight |
73.5 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
266 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWKRLITGLRI |
| Localization (YFP) |
nucleus; spindle
microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |