| Gene name |
SPAC694.02 |
| Gene ID |
48/G12 |
| Gene synonyms/obsolete |
|
| Gene product |
DEAD/DEAH box
helicase; helicase C-terminal domain; no apparent Sc ortholog
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
5154 |
| ORF length (spliced) |
|
| Entry clone length |
5154 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
3026C:T |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC694.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCGAGGAGATAGCCAA |
| Rev primer name |
SPAC694.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCCCACATCTTTCTGAAT |
| Amino acid length |
1717 |
| Molecular weight |
195 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNVDLCKILII/LTCMLEELSL/LGRIKDLNL/LYRIRVLLSL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |