| Gene name |
SPBC29A10.04 |
| Gene ID |
48/E08 |
| Gene synonyms/obsolete |
psm1; smc1 |
| Gene product |
cohesin subunit;
involved in sister chromatid cohesion (required); essential;
interacts physically with Rad21p |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
3702 |
| ORF length (spliced) |
|
| Entry clone length |
3702 |
| No. of intron |
0 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC29A10.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGACGTTTACTAAGACT |
| Rev primer name |
SPBC29A10.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTATTTTAAAATTAAGAAA |
| Amino acid length |
1233 |
| Molecular weight |
141.2 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|