Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1805.04
Gene ID 48/D07
Gene synonyms/obsolete
Gene product nuclear pore complex; involved in nuclear import; involved in nuclear export; involved in nuclear export of the small ribosomal subunit
Entry clone Cloned
ORF length (unspliced) 3553
ORF length (spliced) 3489
Entry clone length 3553
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1805.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTTCTATCCTTGGTAA
Rev primer name SPAC1805.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAATCGTATCGCAACTTAAA
Amino acid length 1162
Molecular weight 132
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLEIYDLKL/LTDALNNYLDL
Localization (YFP) nuclear envelope
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 4 images) See all images

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