| Gene name |
SPAC2F3.10 |
| Gene ID |
47/C10 |
| Gene synonyms/obsolete |
|
| Gene product |
GARP complex; involved
in protein sorting in the late Golgi; involved in retrograde
transport from the prevacuole; involved in docking at the
trans-Golgi network; zf-C2H2 type; zinc finger protein
(inferred from context); involved in actin cytoskeletal
organization; similar to S. cerevisiae YDR027C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2850 |
| ORF length (spliced) |
|
| Entry clone length |
2850 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC2F3.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACGAATTTCATCAGC |
| Rev primer name |
SPAC2F3.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCCGCTTCCTGCTGTTCC |
| Amino acid length |
949 |
| Molecular weight |
107.9 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTDSLQAALTL/LNSRVYQLIL |
| Localization (YFP) |
Golgi; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |