| Gene name |
SPAC22H12.05c |
| Gene ID |
46/F07 |
| Gene synonyms/obsolete |
|
| Gene product |
fasciclin domain
protein (3); cell-adhesion molecule in higher eukaryotes |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2187 |
| ORF length (spliced) |
|
| Entry clone length |
2187 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC22H12.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACCTTCAATTTCGGTT |
| Rev primer name |
SPAC22H12.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACGGATAAAGACGAAGAA |
| Amino acid length |
728 |
| Molecular weight |
82.5 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQFRLYLLFI |
| Localization (YFP) |
vacuole membrane |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |