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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC584.04
Gene ID 46/E02
Gene synonyms/obsolete sup35
Gene product omnipotent nonsense suppressor; EF1 alpha factor-like GTP-binding protein translation release factor; GTP-binding protein; GTP/GDP-dependent (class-II) release factor; omnipotent nonsense suppressor; interacts physically with Sup45p; complexed with Sup45p; altered form creates prions
Entry clone Cloned
ORF length (unspliced) 2072
ORF length (spliced) 1989
Entry clone length 2072
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC584.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAGTAATCAGCCAAA
Rev primer name SPCC584.04.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCAAAATTTTTACGACT
Amino acid length 662
Molecular weight 72.5
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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