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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC15D4.14
Gene ID 46/C03
Gene synonyms/obsolete taf73
Gene product transcription initiation factor TFIID subunit; WD repeat protein; suppresses defects in the anaphase-promoting complex; essential; overexpression suppresses the anaphase blocking mutation cut9; possible role of WD repeat-containing TAFs in the expression of genes involved in progression through the M phase of the cell cycle
Entry clone Cloned
ORF length (unspliced) 1985
ORF length (spliced) 1929
Entry clone length 1985
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC15D4.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTCAATCTTCCTACTC
Rev primer name SPBC15D4.14.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAAGAGCTTAATGCTAGA
Amino acid length 642
Molecular weight 72.2
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRDWIDGTLDL
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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