| Gene name |
SPAC806.02c |
| Gene ID |
46/A10 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat protein; N
terminal is similar to Par A family ATPase; C terminal is
similar to Cia1 family WD repeat domains, this domain
combination does not usually co-occur; gene structure
confirmed; essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1876 |
| ORF length (spliced) |
1827 |
| Entry clone length |
1876 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC806.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTAAATGATATTGAGA |
| Rev primer name |
SPAC806.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTAAAGCTCCAAACGCGA |
| Amino acid length |
608 |
| Molecular weight |
67.6 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |