| Gene name |
SPCC737.03c |
| Gene ID |
46/A04 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
similar to N. crassa NCU03345.1; no apparent Sc ortholog
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1848 |
| ORF length (spliced) |
|
| Entry clone length |
1848 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1836A:deletion /
1837A:deletion / 1838A:deletion |
| Comments |
1 aa deletion |
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC737.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATCTAGCCGATTGTT |
| Rev primer name |
SPCC737.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCCACCTTTCTTAAAGGA |
| Amino acid length |
615 |
| Molecular weight |
70.4 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
6 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPDGIRNLFL/LIIRLTCLYL/LESHFSKSLAL/LLIAFTILFL |
| Localization (YFP) |
ER with
discontinuity |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |