| Gene name |
SPCC1620.12c |
| Gene ID |
45/H04 |
| Gene synonyms/obsolete |
|
| Gene product |
RabGAP domain; TBC
domain protein; GTPase activating protein of Rab-like GTPase;
similar to Sp SPAC26F1.09 (paralog) |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1788 |
| ORF length (spliced) |
|
| Entry clone length |
1788 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1620.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGAAATTAGTTTAGA |
| Rev primer name |
SPCC1620.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTCTAAACATCTCAGAG |
| Amino acid length |
595 |
| Molecular weight |
68.1 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LACCFADSLQL/LNAWVEDSLAL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus |
| Microscope used for
observation |
DeltaVision |